Journal of Tissue Viability
Volume 18, Issue 4 , Pages 109-116 , November 2009

Correlation of donor age and telomerase activity with in vitro cell growth and replicative potential for dermal fibroblasts and keratinocytes

  • M.H. Ng

      Affiliations

    • Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia (National University of Malaysia), Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
    • Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
    • ,
  • B.S. Aminuddin

      Affiliations

    • Ear Nose and Throat Consultant Clinic, Ampang Puteri Specialist Hospital, Selangor, Malaysia
    • ,
  • S. Hamizah

      Affiliations

    • Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
    • ,
  • C. Lynette

      Affiliations

    • Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
    • ,
  • A.L. Mazlyzam

      Affiliations

    • Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia (National University of Malaysia), Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
    • Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
    • ,
  • B.H.I. Ruszymah

      Affiliations

    • Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia (National University of Malaysia), Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
    • Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
    • Corresponding Author InformationCorresponding author. Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia (National University of Malaysia), Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. Tel.: +603 92897224, 91456067; fax: +603 26939687.

References 

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  3. Huang S, Risques RA, Martin GM, Rabinovitch PS, Oshima J. Accelerated telomere shortening and replicative senescence in human fibroblasts overexpressing mutant and wild-type lamin A. Exp Cell Res. 2008;314:82–91
  4. Shay JW, Wright WE. Telomeres and telomerase in aging and cancer. Cold Spring Harbor Monogr Ser. 2008;51:575
  5. Zhou JM, Zhu XF, Lu YJ, Deng R, Huang ZS, Mei YP, et al. Senescence and telomere shortening induced by novel potent G-quadruplex interactive agents, quindoline derivatives, in human cancer cell lines. Oncogene. 2006;25:503–511
  6. Yang L, Suwa T, Wright WE, Shay JW, Hornsby PJ. Telomere shortening and decline in replicative potential as a function of donor age in human adrenocortical cells. Mech Ageing Dev. 2001;122:1685–1694
  7. Shay JW, Wright WE. Forward: ageing and cancer: are telomeres and telomerase the connection. Novartis Found Symp. 2001;235:116–125Discussion 125–129, 146–149. Review
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  12. Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp Cell Res. 1965;37:614–636
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  14. Forsyth NR, Wright WE, Shay JW. Telomerase and differentiation in multicellular organisms: turn it off, turn it on, and turn it off again. Differentiation. 2002;69:188–197
  15. Harle-Bachor C, Boukamp P. Telomerase activity in the regenerative basal layer of the epidermis in human skin and in immortal and carcinoma-derived skin keratinocytes. Proc Natl Acad Sci U S A. 1996;93:6476–6481
  16. Kang MK, Guo W, Park NH. Replicative senescence of normal human oral keratinocytes is associated with the loss of telomerase activity without shortening of telomeres. Cell Growth Differ. 1998;9:85–95
  17. Miyata Y, Okada K, Fujimoto A, Hatae K, Kagami H, Tomita Y, et al. The effect of the long-term cultivation on telomere length and morphology of cultured epidermis. J Dermatol Sci. 2004;34:221–230
  18. Yasumoto S, Kunimura C, Kikuchi K, Tahara H, Ohji H, Yamamoto H, et al. Telomerase activity in normal human epithelial cells. Oncogene. 1996;13:433–439
  19. Taylor RS, Ramirez RD, Ogoshi M, Chaffins M, Piatyszek MA, Shay JW. Detection of telomerase activity in malignant and nonmalignant skin conditions. J Invest Dermatol. 1996;106:759–765
  20. Shealy FG, DeLoach ED. Experience with the use of apligraf to heal complicated surgical and nonsurgical wounds in a private practice setting. Adv Skin Wound Care. 2006;19:310–322
  21. Porter PC, Clark DR, McDaniel LD, McGregor WG, States JC. Telomerase immortalized human fibroblasts retain UV-induced mutagenesis and p53-mediated DNA damage responses. DNA Repair (Amst). 2006;5:61–70
  22. Wege H, Chui MS, Le HT, Strom SC, Zern MA. In vitro expansion of human hepatocytes is restricted by telomere-dependent replicative aging. Cell Transplant. 2003;12:897–906

PII: S0965-206X(09)00034-5

doi: 10.1016/j.jtv.2009.06.003

Journal of Tissue Viability
Volume 18, Issue 4 , Pages 109-116 , November 2009

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